Mustafa, Isshadiba Faikah; Hussein, Mohd Zobir; Idris, Abu Seman; Ramli, Nur Rashyeda; Mustafa, Muskhazli; Fakurazi, Sharida

The endophytic bacterium, Pseudomonas aeruginosa, was successfully encapsulated into calcium carbonate microshells and coated with sodium alginate in combination with two other materials: skim milk and empty fruit bunch (EFB). The presence of bacteria cells was confirmed by a strand-like structure, a biofilm through morphology and elemental analysis.

The survivability of microencapsulated bacteria was found to last for 17 months when they were maintained in a storage condition of 4 °C. Different coating materials used exhibited significant differences in the P. aeruginosa survival during the storage time. Their bioactivity against Ganoderma boninense resulted in a percentage inhibition radial growth (PIRG) value of more than 70%, which is better than its counterparts, the free Pseudomonas cells. With promising viability results of × 106 CFU/mL after three-month storage, the results demonstrate that skim milk-coated alginate might be good protection for P. aeruginosa that could sustain the viable bacteria at the target site. This is toward a greener, biological control-based plant protection for Ganoderma diseases in the oil palm planting industry.

Keywords: Calcium Carbonate; Granules; Microencapsulation; Pseudomonas aeruginosa; Survival

Please find the full article at:

Mustafa, I.F., Hussein, M.Z., Idris, A.S. et al. Pseudomonas aeruginosa encapsulated with calcium carbonate microshells for potential biocontrol of the Ganoderma boninense. Korean J. Chem. Eng. 40, 854–862 (2023). https://doi.org/10.1007/s11814-022-1351-5 .

Date of Input: 16/07/2024 | Updated: 16/07/2024 | roslina_ar